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KMID : 1161520090130020097
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Animal Cells and Systems
2009 Volume.13 No. 2 p.97 ~ p.103
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Considering cell-based assays and factors for genome-wide high-content functional screening
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Chung Chul-Woong
Kim In-Ki
Jung Yong-Keun
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Abstract
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Recently, great advance is achieved in the field of genome?wide functional screening using cell?based assay. Here, we briefly introduce well?established and typical cell?based assays of GPCR and some parameters which should be considered for genome?wide functional screening. Because of characters and importance of GPCR as drug targets, several ways of assay systems were devised. Among them, high?content screening (HCS) that is based on the analysis of image by confocal microscope is becoming favorite choice. The advances in this technology have been driven exclusively by industry for their convenience. Now, it is turn for academy to define more detail signaling networks via HCS using cDNA or siRNA libraries at genome?wide level. By isolating novel signaling mediators using cDNA or siRNA library, and postulating them as new candidates for therapeutic target, more understanding about life science and more increased chances to develop therapeutics against human disease will be achieved.
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KEYWORD
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cell-based assay, screening, siRNA library, cDNA library
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